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Carnegie Stage 17

The movies below show volume renders of OPT models. No staining has been used on any of the specimens and the images were captured using autofluorescence with a FITC filter.

N363 46XX	N365 46XX	N366 46XY
N471 46XX	N478 46XY	N512 46XY
N522 46XX	N544 46XY	N571 46XX

 

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Carnegie Stage 17

The movies below show anatomical domains defined in the N365 model. The domains have been manually defined in the OPT model using specialised software and then visualised using commercial rendering packages.

The various domains are: heart - red; liver - dark green; eye - dark blue; otic vesicle - yellow; dorsal root ganglia - cyan. The CNS domains have been further subdivided as follows: pallium - red, subpallium - orange, hypothalamus - light brown, prosomere 3 - bright green, prosomere 2 - green, prosomere 1 - dark green; mesencephalon - blue; isthmus - dark purple, metencephalon (rhombomeres 1-6) - purples/pinks; myelencephalon (rhombomeres 7-11) - magenta; spinal cord - maroon.

The top panel is a surface render of the 3D anatomical regions, and the bottom panel shows the painted N365 model being digitally sectioned through the z-plane.

 

 

 

 

 

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Carnegie Stage 17

The movies below show rendered representations of immunohistochemistry data from sections through the head of N365 which have been digitised, thresholded out and placed back onto the OPT model of N365.

PAX6	GAP43	Beta-tubulin

 

PAX6: The staining is shown in red. For reference the eyes have been shown in dark grey, and the PAX6-negative regions of the neural tube are shown in light grey.

GAP43: Here the individual cranial nerves have defined and painted as follows: Olfactory (I) - light blue, Oculomotor (III) - yellow/green, Trochlear (IV) - blue/green, Trigeminal (V) - violet, Abducens (VI) - deep orange, Facial (VII) - pink, Vestibulocochlear (VIII) - white, Glossopharyngeal (IX) - yellow, Vagus (X) - red, Accessory (XI) - bright green, Hypoglossal (XII) - light orange and spinal nerves bright blue. Only one hemisphere is shown to allow visualisation of the nerves from both sides. For reference the eye is painted dark grey and the neural tube light grey.

B Tubulin: The strong staining is shown in red while the orange represents the weaker staining. The eyes have been shown in dark blue for reference purposes.

 

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Carnegie Stage 17 Definition

 

Age is approximately 41 postovulatory days Length is approximately 11-14mm External Features Include: the hand plate exhibits definite digital rays; the foot has acquired a rounded digital plate; nasofrontal grooves are distinct. O'Rahilly R and Muller F [1987] Developmental Stages in Human Embryos. Carnegie Institute, Washington, Publication no. 637)