• Volume render movies
  • Painted anatomical regions
  • Carnegie Stage Definition

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Carnegie Stage 19

The movies below show volume renders of OPT models. No staining has been used on any of the specimens and the images were captured using autofluorescence with a FITC filter.

 

 
N454 46XY
 
N534 46XY

 

 
N337 46XY
 
N344 46XX

 

 

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Carnegie Stage 19

The movies in the left and centre panels are surface renders of neuroanatomical domains. These have been manually defined in the N454 CS19 OPT model using specialised software and then visualised using commercial rendering packages.

 

     
N454 46XY
N454 46XY
N454 46XY

 

Left: The domains are neural tube - orange; heart - red; liver - green; eye - dark blue; otic vesicle - yellow and dorsal root ganglia - light blue.

Centre: The domains are eye - light grey; pallium - red, subpallium & hypothalamus - orange; prosomere 3 - yellow, prosomere 2 - bright green, prosomere 1 - dark green, mesencephalon - blue; metencephalon - purple; myelencephalon - pink; spinal cord - maroon.

The movie on the right shows the same N454 model and painted anatomical domains being digitally sectioned through the z-plane. The various domains are: heart - red; liver - dark green; eye - dark blue; otic vesicle - yellow; dorsal root ganglia - cyan. The CNS domains have been further subdivided as follows: pallium - red, subpallium - orange, hypothalamus - light brown, prosomere 3 - bright green, prosomere 2 - green, prosomere 1 - dark green; mesencephalon - blue; isthmus - dark purple, metencephalon (rhombomeres 1-6) - purples/pinks; myelencephalon (rhombomeres 7-11) - magenta; spinal cord - maroon.

 

 

 

 

 

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Carnegie Stage 19 Definition

 

Age is approximately 47-48 postovulatory days

Length is approximately 17-20 mm

External Features Include: the trunk has begun to straighten and elongate slightly, with the result that the head no longer forms a right angle with the line of the back of the specimen, the limbs extend nearly directly forward; the toe rays are more prominent, but interdigital notches have not yet appeared in the rim of the footplate.

O'Rahilly R and Muller F [1987] Developmental Stages in Human Embryos. Carnegie Institute, Washington, Publication no. 637)