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Carnegie Stage 22

The movie below shows a micro-CT scan of a CS22 embryo. Sample staining and micro-CT was performed by Newcastle Preclinical In Vivo Imaging (PIVI) facility. 3D data-sets were acquired using Skyscan 1176, Bruker-microCT.

14916, male. This embryo has a cleft palate.

 

The movies below show volume renders of OPT models. No staining has been used on any of the specimens and the images were captured using autofluorescence with a FITC filter.

N419 head 46XY	N419 body 46XY	N542 46XX

 

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Carnegie Stage 22

The movies below show anatomical domains defined in the N542 model. The domains have been manually defined in the OPT model using specialised software and then visualised using commercial rendering packages.

The various domains are: eye - dark blue; otic vesicle - yellow; dorsal root ganglia - cyan. The CNS domains have been further subdivided as follows: pallium - red, subpallium - orange, hypothalamus - light brown, prosomere 3 - bright green, prosomere 2 - green, prosomere 1 - dark green; mesencephalon - blue; isthmus - dark purple, metencephalon (rhombomeres 1-6) - purples/pinks; myelencephalon (rhombomeres 7-11) - magenta; spinal cord - maroon.

The top panel is a surface render of the 3D anatomical regions, and the middle panel shows the painted N542 model being digitally sectioned through the z-plane.

 

The movie below is a another surface render of anatomical domains within the brain of the CS22 model. Here the floor of the brain has been painted red, and the roof blue. For reference the rest of the neural tube is painted transparent grey.

 

 

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Carnegie Stage 22 Definition

 

Age is approximately 54 postovulatory days Length is approximately 25-27mm External Features Include: the hands extend further in front of the body and the fingers of one hand may overlap those of the other. The superficial vascular plexus of the head extends upward to form a line at approximately 3/4 of the way above the eye-ear level. O'Rahilly R and Muller F [1987] Developmental Stages in Human Embryos. Carnegie Institute, Washington, Publication no. 637