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Queen et al. PLOSGENETICS-D-23-00517
Below are RNAscope and immunofluorescence data for novel genes identified by spatial transcriptomic analysis of arterial valves at CS16 and CS19. H&E sections from a mouse model of one of these genes (RBP1) show structural heart defects.
Click on the images below to enlarge
Human Embryos
CS16 ST section
CS19 ST section
CS16 CRABP2 IF
CS16 LGALS1 IF
CS16 ID4 RNAscope
CS16 HES4 RNAscope
CS16 MXRA5 RNAscope
CS16 PDLIM3 RNAscope
CS16 STX10 RNAscope
CS16 EIF2A RNAscope
CS16 RBP1 RNAscope
CS19 EIF2A/HOTAIR RNAscope
CS19 RBP1 RNAscope
CS20 RBP1 RNAscope
Mouse
E11.5 CRABP2 IF
_02_c1+2_Hannah_set_2-th.jpg)
E11.5 RBP1 IF
_03_c1+2 Hannah set 2.jpg)
E12.5 LGALS1 IF
 E12.5 T Embryo 3-10_c1+2 (1) Scale Bar-th.jpg)
E12.5 RBP1 IF
_33_c1+2-th.jpg)
E13.5 RBP1 IF
_04_c1+2-th.jpg)
WT mouse E15.5 H&E
WT mouse E15.5 H&E
WT mouse E15.5 H&E
WT mouse P1 H&E, sectioned head to rear
WT mouse P1 H&E, sectioned head to rear
WT mouse P1 H&E, sectioned head to rear
WT mouse P1 H&E, sectioned head to rear
WT mouse P1 H&E, sectioned rear to head
WT mouse P1 H&E, sectioned rear to head
WT mouse P1 H&E, sectioned rear to head
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-3)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-3)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-3)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-2)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-2)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-2)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-2)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-2)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-4)
RBP1 KO mouse E15.5 H&E, sectioned tail to head (872-4)
RBP1 KO mouse P1 H&E, sectioned head to tail (851-2)
RBP1 KO mouse P1 H&E, sectioned head to tail (851-2)
RBP1 KO mouse P1 H&E, sectioned head to tail (851-2)
RBP1 KO mouse P1 H&E, sectioned head to tail (851-2)
RBP1 KO mouse P1 H&E, sectioned head to tail (851-2)
RBP1 KO mouse P1 H&E, sectioned head to tail (851-2)
_02_c1+2_Hannah_set_2.jpg)
_03_c1+2_Hannah_set_2-th.jpg)
 E12.5 T Embryo 3-10_c1+2 (1) Scale Bar.jpg)
_33_c1+2.jpg)
_04_c1+2.jpg)
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