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Gene Expression defining boundaries in the brain at CS19

Here data from immunohistochemical experiments has been compared with the digitally painted CS19 model. When the sections are aligned it becomes evident that that the gene expression patterns define boundaries between different anatomical regions:

Black arrowhead - diencephalon/telencephalon boundary.

Black arrow - pallial/subpallial boundary.

White arrowhead - boundary between lateral and medial ganglionic eminences.

White arrow - boundary between the prethalamus (prosomere 3) and the hypothalamus.

Black lines - boundary between the alar and basal plates in the hindbrain.

Click on the gene expression images for a higher magnification view.

  

 

Gene expression in the human embryonic brain Gene expression in the human embryonic brain Gene expression in the human embryonic brain Gene expression in the human embryonic brain
GAP43
PAX6
OLIG2
Transverse view of the painted model
     
Gene expression in the human embryonic brain Gene expression in the human embryonic brain Gene expression in the human embryonic brain Gene expression in the human embryonic brain
TBR1
GAD2
NKX2-2
Sagittal view - section plane is indicated by the line
 

Key to painted anatomy:

Pallium, Lateral Ganglionic Eminence, Medial Ganglionic Eminence, Preoptic and Diagonal Band Areas, Hypothalamus, Prosomere 3, Prosomere 2, Prosomere 1, Midbrain, Isthmus, Rhombomere 1, Rhombomere 2, Rhombomere 3, Rhombomere 4, Rhombomere 5, Rhombomere 6, Rhombomeres 7-11, Spinal Cord.