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Gene Expression defining boundaries in the brain at CS22

  

Here data from immunohistochemical experiments has been compared with the digitally painted CS22 model. When the sections are aligned it becomes evident that that the gene expression patterns define boundaries between different anatomical regions:

Black arrowhead - diencephalon/telencephalon boundary.

Black arrow - pallial/subpallial boundary.

White arrowhead - boundary between lateral and medial ganglionic eminences.

White arrow - boundary between the prethalamus (prosomere 3) and the hypothalamus.

Black lines - boundary between the alar and basal plates in the hindbrain.

Click on the gene expression images for a higher magnification view

   
gene expression in the human embryonic brain gene expression in the human embryonic brain gene expression in the human embryonic brain
PAX6
OLIG2
Transverse view of the painted model
   
gene expression in the human embryonic brain gene expression in the human embryonic brain gene expression in the human embryonic brain
GAD2
TBR1
Sagittal view - section plane is indicated by the line
 

Key to painted anatomy:

Pallium, Lateral Ganglionic Eminence, Medial Ganglionic Eminence, Preoptic and Diagonal Band Areas, Hypothalamus, Prosomere 3, Prosomere 2, Prosomere 1, Midbrain, Isthmus, Rhombomere 1, Rhombomere 2, Rhombomere 3, Rhombomere 4, Rhombomere 5, Rhombomere 6, Rhombomeres 7-11, Spinal Cord.